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Image Search Results
Journal: Cancer Research
Article Title: Repression of IFN Regulatory Factor 8 by DNA Methylation Is a Molecular Determinant of Apoptotic Resistance and Metastatic Phenotype in Metastatic Tumor Cells
doi: 10.1158/0008-5472.can-06-4068
Figure Lengend Snippet: Figure 5. The 5¶ regulatory region of the IRF8 gene is methylated. A, genomic organization of the human IRF8 gene promoter region. The 5¶ regulatory region of the human IRF8 gene was deduced from the human genome DNA sequence. Organization of the 5¶ untranslated region (exon 1) and 5,000 bp (5,000) upstream from the putative transcription initiation site (+1). Solid dark gray box, location of the CpG island; open box, consensus GAF-binding element (GAS). B, methylation-sensitive PCR analysis of the IRF8 gene promoter region in the primary colon carcinoma SW480 cells and the metastatic colon carcinoma SW620 cells. Genomic DNA was isolated from these two tumor cell lines and modified using the CpGenome Universal DNA Modification kit as described in Materials and Methods. The modified genomic DNA was then used as template for methylation-sensitive PCR analysis using unmethylated (U) or methylated (M) primers.
Article Snippet: The
Techniques: Methylation, Sequencing, Binding Assay, Isolation, Modification
Journal: Oncotarget
Article Title: The generation and analyses of a novel combination of recombinant adenovirus vaccines targeting three tumor antigens as an immunotherapeutic
doi:
Figure Lengend Snippet: SW620 tumor cells were used as positive control. Actin was used as a loading control. A. Expression of brachyury was robust in DCs infected with Ad5 [E1-, E2b-]-brachyury. B. MUC1 expression was observed in human DCs infected with Ad5 [E1-, E2b-]-MUC1 vector as compared to DCs infected with Ad5 [E1-, E2b-]-null (no transgene).
Article Snippet:
Techniques: Positive Control, Control, Expressing, Infection, Plasmid Preparation
Journal: Oncotarget
Article Title: The generation and analyses of a novel combination of recombinant adenovirus vaccines targeting three tumor antigens as an immunotherapeutic
doi:
Figure Lengend Snippet: Infection of human DCs with Tri-Ad5 can generate brachyury-, MUC1- and CEA-specific CTLs that efficiently lyse tumor cells expressing all three antigens
Article Snippet:
Techniques: Infection, Expressing